Makful, S. Purnomo, and Sunyoto. Analysis of Genetic Diversity of Mangosteen Basedon the Amplified Fragment Length Polymorphism (AFLP) Technique. For estimating genetic distance the AFLP and RFLP markers gave the most The dominant markers (AFLP and RAPD) had small CV values indicating a. Key Words: Aegilops, AFLP, DNA fingerprinting, durum wheat. Bu¤dayda ve Aegilops’ta çok teknik deneyim gerektirmektedir. Bununla birlikte ÇPUP.
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Although similar average genetic distance values were obtained for the BR and BR intrapopulation crosses, the BR crosses showed the widest range of genetic distances with all of the four different markers assayed; probably because of the broader genetic base of the BR population.
Correlation of genetic distance with F 1 grain yield and heterosis. The application of SSR techniques to plants depends on the availability of suitable microsatellite markers, which have been developed for species such afllp soybean Rongwen et al.
Afpl results obtained in our study showed high agreement between RFLP and AFLP genetic distance estimates, such estimates having also been highly correlated in other studies Russell et al. The Plant J 3: Comparisons with data from RFLPs and pedigree.
The information content of each marker system was calculated for each marker and locus using the polymorphism information content PIC Lynch and Walsh, which provides an estimate of the discriminating power of a locus teonii taking into account not only the number of alleles that are expressed but also their relative frequencies.
AFLP also correlates highly with results obtained using the RFLP system and is a fast and reliable system capable of supporting a multiplex approach not requiring previous knowledge of DNA sequencing. The AFLP system presents good levels of precision in its genetic estimates and single teonii prediction.
Analisis Keragaman Genetik Manggis Menggunakan Teknik Amplified Fragment Length Polymorphism (AFLP)
The results also suggest that the number of loci evaluated should be increased. Pesq Agropec Bras 28, The choice of the appropriate number of polymorphic loci required for a reliable estimation of genetic distance is influenced by the criteria used, and it appears that the maximum and median CV values are the best choice for evaluating the precision of the genetic distance estimates based on molecular marker data sets.
All of the 18 maize inbred lines studied aflo us had previously been investigated using the four different marker systems RAPD: Data analysis Both dominant markers RAPD and AFLP were used to calculate the genetic distances between the 18 inbred lines using the complement of the Jaccard’s similarity coefficient Jaccard, which takes into account the presence or absence of bands.
Although the discrimination power of RFLPs in diversity studies has been well documented Smith et al. The loci used were: Both dominant markers RAPD and AFLP were used to calculate the genetic distances between the 18 inbred lines using the complement of the Jaccard’s similarity coefficient Jaccard, which takes into account the presence or absence of bands. V general method for DNA teknio. Plant Breeding teknli Services on Demand Journal. Similar patterns were observed for both genetic distance and heterosis.
For the AFLP method 20 primer combinations were used and binary scored 1 or 0 with each band being considered a locus while aaflp the SSR method 68 polymorphic primers were used with the binary data being converted into a genotypic matrix which was used to identify alleles and their teknli loci.
However, we also found that SSRs markers gave a more heterogeneous distribution for individual PIC values than RFLP markers, although this might have been due to the low number of polymorphic loci evaluated for this tknii Barbosa et al.
The coefficient of variation CV for all genetic distances across the tekniu samples was estimated for geknii specific number of loci or bands sampled, a computer program for performing these analyses being set up using the ‘RANNUNI’ function of the SAS system Version 8.
Ag Biotech News Inf 6: Tekii in Chinese sorghums. The RFLP system appears to be the most robust marker assay in terms of the amount of polymorphism surveyed, although, in practice, it is still a very laborious technique. Jaccard similarities were calculated using version 2. Differences in the distribution profiles also occurred between dominant and codominant markers, with dominant markers having higher standard deviations than codominant markers.
The correlation coefficient values between genetic distance and hybrid performance for the four markers assayed were similar to the correlation values between genetic distance and heterosis, not only for the inter or intrapopulation crosses but also for all crosses combined. Correspondence to Antonio A. Bull Soc Vaud Nat Bootstrap analysis were used to evaluate tejnii number of loci for the markers and the coefficients of variation CV revealed a skewed distribution.
J Genet Breed Even though the CV values were not low enough to indicate a high level of precision the SSR markers produced high, and the RAPD markers moderate, correlations between the genetic distance estimates and hybrid performance and heterosis for the BR intrapopulational crosses.
Brazilian breeding programs have exploited the genetic diversity of the BR population and demonstrated that high performance cultivars can be obtained from this population Gerage et al. In this method, co-occurrences are divided by the total number of evaluated loci excluding the negative falp and thus can be interpreted as the proportion of sflp in relation to tekniu total number of evaluated loci. Detailed descriptions of these populations are given in Lanza et al. Heterotic group assignment and hybrid performance determined by RFLP marker.
Mol Gen Genet Pearson’s correlation coefficient was calculated for the genetic distances, single cross performance and heterosis as previously described by Benchimol et al. Comparisons of different DNA markers for diversity studies in maize Hahn et al. Morgante and Olivieri stated that in soybean the amount of information given by SSR loci in relation to a comparable number of RFLP loci is given by the estimated number of alleles 4. Materials and methods Plant material and DNA isolation Eighteen S 3 selected inbred lines from two divergent tropical maize populations eight from BR and ten from BR previously had their genetic distances surveyed using four different marker systems Lanza et al.
Total genomic DNA was isolated from a bulk of five-week-old leaf tissue taken from 16 plants of each line, then being isolated and purified by the method of Hoisington et al.
To determine the sampling variance of the genetic distances produced by the different molecular data sets we performed bootstrap analysis using a decreasing number qflp loci for codominant markers or bands for dominant markers. Theor Appl Genet Barbosa I ; Isaias O.
Thirty-two primers showing reproducible polymorphism were selected and used for scoring the 18 inbred lines. We used the median and maximum coefficient of variation values to evaluate the accuracy of the genetic distance estimates because although the mean coefficient of variation is often used in the literature caution is needed when dealing with molecular marker data for which there is no assurance that the CVs values are distributed symmetrically.
VI Isozyme variation among races of maize in Bolivia.
Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines
Our results indicate that AFLP seemed to be the best-suited molecular assay for fingerprinting and assessing genetic relationships among tropical maize inbred lines with high accuracy. The results obtained based on the adjusted functions except for the mean CV shown in Figure 3 are presented trknii Table 3.
Indeed, we found that the RFLP and AFLP markers produced sufficient numbers of polymorphic bands to produce reliable genetic distance estimates with high correlations between these two marker systems, the similarity between the results being explainable by the fact that they are similar techniques based on restriction site changes.
A new technique for DNA fingerprinting.